ANFS332 Lecture 10: Enterobacteriaciea, Escherichia coli O157H7, colibacillosis

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11:10 am: red text = important, blue text = do not need to know. Bacterial identification o o o o o o o o o o. If more than one colony type is present then subcultures are made. Growth on blood based agar for hemolytic reactions. Hydrogen sulfide production (h2s) results in a black color. Facultative anaerobes the patterns of fermentation are often used to differentiate them. Over 30 genera and 120 species many are part of the normal bacteria flora of the gastrointestinal tract. Some are primary pathogens if you see them in an organism you know they will cause disease (ex salmonella) Some are opportunistic pathogens they can cause disease under certain conditions or in certain hosts (ex e coli) A diagnostic method which characterizes the biochemical activities of an organism. The first test is for the presence of the enzyme b-galatosidase, an enzyme involved in lactose catabolism.

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