BISC300 Lecture Notes - Lecture 13: Polymerase Chain Reaction, Human Papillomavirus Infection, Molecular Cloning
27 Nov 2016
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Deli(cid:271)erate (cid:373)odifi(cid:272)atio(cid:374) of orga(cid:374)is(cid:373)"s ge(cid:374)eti(cid:272) i(cid:374)fo (cid:271)y dire(cid:272)tly (cid:272)ha(cid:374)gi(cid:374)g the sequence of nucleic acids in its genome: recombinant dna (rdna) technology. Procedures used to carry out genetic engineering: cloning. Generation of a large # of genetically identical dna molecules. Restriction enzymes: recognize and bind specific sequences in dna called recognition sites, cleave dna at this site or a defined distance from it, may produce sticky ends or blunt ends in dna target, hundreds are commercially available. Blunt ends are harder to match up than sticky ends. Polymerase chain reaction (pcr: enables gene amplification. Rapid synthesis of many copies if a specific dna fragment from a complex mixture of dna and other cellular components. Chain length can vary from short (2-30 nucleotides) to long (50- Uses of pcr: simplifies gene cloning, generates dna fragments for nucleotide sequencing, may amplify enviro genes w/o culturing microbes, diagnostic purposes. Aids, lyme disease, chlamydia, tuberculosis, hepatitis, human papilloma virus. Pcr reaction mix: reaction mix contains.
