BISC439 Lecture Notes - Lecture 19: Ventricular Zone, Retinal Ganglion Cell, Okazaki Fragments
6 Apr 2017
School
Department
Course
Professor
Document Summary
Developmental neuro paper 7 notes: lmpcr is much more sensitive than normal pcr; amplifies dna exponentially, fig. 1 thymus is known to undergo pcd: clusters of dying t cells that are overreactive and could be toxic, dexamethasone induces apoptosis, d-f: retinal ganglion cells postnatally. Isel+ is labeling cell death: amount of cell death decreases with label intensity (pd1 pd5 pd9, fig 2. Isel+ can stain with many types of apoptotic inducers: apoptosis induced by uv light and labeled with isel, embryonal carcinoma line p19 pluripotent stem cells, fig. 3: a: control section of e16 mouse brain (control, naturally occurring pcd, lmpcr would show ladder pattern, b: tdt enzyme denatured no visible pcd cells, c: incubated section c in rnase (to denature rna) Isel+ doesn"t stain rna: d: isel+ can access every single nuclei, dnase: fragments the dna, the reason you didn"t see all cells stained in panel a is just because the.
