CBIO 3400 Lecture Notes - Lecture 1: Organelle, Ribosome, Nucleolus

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Nucleus: main characteristics: membrane-enclosed organelle found in eukaryotic cells, generally, found in the central region of the cell (in animal cells, roughly spherically shaped, largest and most easily seen organelle. Primary functions within the cell: repository of genetic information (dna & rna, responsible for production of ribosomes, enables the synthesis of nearly all proteins. What is the benefit of doing this: the spatial and temporal separation of transcription and translation enables eukaryotes to regulate gene expression in much more intricate ways, contributing to the richness of eukaryotic form and function. Within the nucleolus there are sub-regions: fibrillar center: where rrna transcription occurs, dense fibrillar component: after transcription, where pre-rrna processing takes place, granular component: location of pre-ribosome assembly. Transcription factory: an evolution of understanding: fluorescently label rna pol ii and use superresolution microscopy to examine individual. Rna pol ii molecules: most rna pol ii molecules are solitary and are too far away from others to constitute a.

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