MCB 250 Lecture Notes - Lecture 8: Nucleoside Triphosphate, Phosphodiester Bond, Deoxyribonucleoside
9 Jan 2020
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Scale of the problem: e. coli chromosome is 4. 5 x 106 base pairs and is 1mm long. If the dna was a 2-stranded kite strung 1mm wide, then it would be mile long and the cell would be. Of a meter in diameter: your assignment: separate the two strands, wrap a new strand around each and end up with two new pieces and no knots. You have 40 minutes with an average error rate (incorporation of a wrong base, i. e. a mutation) of once per 109 bases. Dna replication is semiconservative: the products of replication are duplexes with one old strand (blue) and one new strand (red) Dna is synthesized by extending the 3" end of the primer: the chemistry of dna synthesis requires that the new chain grows by extending the 3" end of the primer, the phosphodiester bond is formed in an.
