MCB 250 Lecture Notes - Lecture 14: Tautomer, Depurination, Pyrimidine

• School: University of Illinois
• Department: Molecular and Cell Biology
• Course: MCB 250
• Professor: J Slauch

Document Summary

Introduction: mutant, an organism that differs in dna sequence from the wild type, mutation, a stable, heritable change in the dna sequence, can be spontaneous or induced, mutagen, a physical or chemical agent that induces mutations. Point mutations: transition: purine to purine, pyrimidine to pyrimidine to pyrimidine, more common than transversion mutations, transversion: purine to pyrimidine, pyrimidine to puring. Point mutations consequences for proteins: silent mutation, missense mutation, nonsense mutation. Mutation notation: mutations are commonly designated by: wild type amino acid position in the protein mutant amino acid, for example: e214k, the mutation resulted in a glu at amino acid position 214 changed to lys. Spontaneous mutation: errors in dna replication, may result from incorporation errors perhaps caused by tautomerization, may also result from misalignment, chemical instability of dna, deamination of c, depurination, oxidative dna damage, modified bases, single and double stranded breaks.