MCB 250 Lecture Notes - Lecture 25: P Element, Molecular Cloning, Transgenesis
9 Jan 2020
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Document Summary
Introduction: with the advent of dna cloning technology in the 1970s-1980s, it became possible to modify or. Introduction: the methods used to introduce transgenes vary widely between different species, one of the first (and still most effective) methods was developed in the fruit fly and relies upon a type of. Dna transposon called the p element: p elements were discovered in the 1970s in a wild population of drosophila melanogaster called the p strain. The transposase gene has been replaced with cloned gene x and a marker gene (w+) The wild-type white (w+) gene is responsible for the red pigment of the fly"s compound eye. Flies that are homozygous loss-of-function for this gene (w /w ) have white eyes: plasmid #2 does not have a p element. Some germ cells will receive one or more p element insertions; other germ cells will have none: the germline of the injected fly is a mixture of cells with and without p elements.
