BIOLOGY 305 Lecture Notes - Lecture 6: Enterobacteria Phage P22, Enterobacteria Phage T2, Centimorgan
Document Summary
Bacterial and viral genetics: crossing an hfr strain with f- F-: leu+, ser+, mal+, suc+, gal-, amps leu-, ser-, mal-, suc-, gal+, ampr. Before your un-interrupted conjugation experiment, you need to make media plates that select for certain markers. Supplements leu ser mal suc gal glu amp. After 10 minutes of uninterrupted mating, 100 ul of the conjugation mixture above was spread onto media 1, 2, and 3. How do you calculate cotransduction frequencies: lytic v. lysogenic, a cross is made between the streptomycin resistant (sr) f--- strain marked with the characters. Gal--- t---azr lac---tlr mal---xyl---l--- and the prototrophic hfr strain having opposite characters. After 60 minutes of contact, samples are transferred to plates with minimal medium plus streptomycin. All markers can be transferred within this time. Key to characters: t and l are amino acids; gal, lac, mal, xyl are sugars; az = azide; t1 is a phage; s = streptomycin.
