MCDB 310 Lecture Notes - Lecture 18: Citric Acid Cycle, Urea Cycle, Leucine

• School: U of M
• Department: Molecular, Cellular and Developmental Biology
• Course: MCDB 310
• Professor: Kenneth Balazovich

Document Summary

Mcdb lecture 18: amino acid degradation & synthesis (chapter 18 & 22) Learning goals: how are nitrogen-containing molecules transported and excreted, mechanism of an enzyme, aminotransferase, the urea cycle, link between the urea cycle and the citric acid cycle. We need an organized strategy to dispose of nitrogen: solution, start with proteins, make them into amino acids, then we separate the energy rich carbon skeletons from the nitrogen (splitting aa into ammonium. Carbon skeletons (including r groups) goes to the citric acid cycle and ammonium goes to the urea cycle. Amino acid oxidation and the citric acid cycle: urea cycle and the citric acid cycle are connected, all 20 amino acids can feed into the cac, ketogenic: go to acetyl-coa & ketone bodies, acetyl-coa. Isoleucine: leucine, tryptophan, acetyl-coa via acetoacetyl-coa, leucine, lysine, phenylalanine, tryptophan, tyrosine, glucogenic: directly into cac as intermediates, as pyruvate, alanine, cysteine, glycine, serine, tryptophan, as oxaloacetate, asparagine, aspartate (glucose, as fumarate, phenylalanine, tyrosine, as succinyl-coa.