FRAP, PHotoactivation, BiFC

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Department
Biochemistry & Molecular Bio.
Course
BIOCHEM 524
Professor
David Gross
Semester
Spring

Description
March 7 Notes • FRAP to detect movement & dynamics o photo-bleach portion of already fluorescent cell, causes non-fluorescence o can recover the fluorescence of photo-bleached region to detect movement • Photoactivation to detect movement & dynamics o region within cell can be activated by light o activate region and look @ movement & dynamics • Forster (fluorescence) resonance energy transfer (FRET) o donor chromophore absorbs light @ certain wavelength, becomes excited o can
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