BIOS 111 Lecture Notes - Lecture 4: Bromothymol Blue, Gram-Negative Bacteria, Ph Indicator
Document Summary
When an enzyme is denatured, its entire structure comes apart and inactivates the protein"s functions. Occasionally, proteins can be denatured and then refolded back into its original shape: explain the relationship of a microbe"s maximum growth temperature and proteins. A microbe"s maximum growth temperature is predetermined by its protein"s stability if the temperature of an environment rises above the maximum growth temperature, a protein will denature and the microbe will die. Sim tubes are used to test for a microbe"s ability to produce hydrogen sulfide in gaseous form: in the sim tests, list the three hydrolases and the products of each reaction in which they are involved. Cysteine desulfurase has the end products of 2c3h4o3, 2nh3, and h2s. Thiosulfate reductase, another hydrolase of sim testing, has the end products of 2so3 and 2h2s. The final hydrolase, indole, has the end products of pyruvic acid and ammonia.
