BIOL 483 Lecture Notes - Lecture 33: Nicotinamide Adenine Dinucleotide, Histone H1, Embryonic Stem Cell
Document Summary
Not known what deposits it but some remodelers prevent its deposition. Has macro domain on top of canonical h2a domains. > since rest of histone structure is similar to h2a -> well conserved structure. Has long c terminal extension (x3 h2a size) Binds metabolites of nad+ that are tightly linked to organism"s metabolism (eg. adp-ribose, > macroh2a1 can be alternatively spliced to generate splice variants (macroh2a1. 1, macroh2a1. 2) Macro domain roles: signalling domain in response to metabolites of nad+ interacts with proteins found in compact chromatin (hp1, histone h1) Macroh2a roles: chromatin compaction (especially during x inactivation) by interacting with prc2, overlap between histone modifications generated by prc2 (h3k27me3, hira (histone h3. 3 chaperone) assist directly/ indirectly in deposition of macroh2a, atrx removes macroh2a, repressive roles. Inhibit chromatin remodelling by swi/snf a: compaction, stabilisation, prevents binding of transcriptional activators and repressors, repressive nuclear condensates i) ii) Condensate formation and stabilisation of chromatin prevents binding of.
