BIOL 483 Lecture Notes - Lecture 60: Insulin-Like Growth Factor 2, Ctcf, Northern Blot
Document Summary
Knockin mouse when you look at sperm from knockin mouse, you can see sperm is not properly methylated. What happens is when you look at 2 cell embryos, what little methylation present in embryo is gone. This particular allele then results in biallelic h19 and complete loss of igf2. We use this to understand what is different from allele to not be methylated in mouse germline. How does methylation find its way on icr in male germline. What is about it that causes h19 and icr and delta icr to be methylated in male germline. Want to use mouse line to see if it could be recovered. If you overexpress h19 -> get some phenotypes. Loss of 2 igf2 with h19 -> lethality phenotype that is being pushed forward by h19. Have too much h19 so cross with new mouse with deleted h19 and this came from mum and crossed it to males with hic1.
