CHEM 251 Lecture Notes - Lecture 12: Aminoacyl-Trna, Lac Repressor, Stop Codon
Document Summary
Mrna transcripts code for one/ couple of proteins, mrna transcripts can be read from either strand of dna/ polymerases act on both strands to generate rnas. Rna pol is like dna pol (ntp comes in and forms watson crick interactions between template. Once pol gets going, sigma subunit falls off/ industrial protein falls off/ keeps polymerase engaged. Terminator sequence, once rna of terminator sequence made -> hairpin/ self pair -> rna pol falls off. Nucleic acid with corresponding protein seq, it"s the job of trna. Get into a site by mimicking aminoacyl trna structures. Crystal structures of ef-g structures, ef-tu + trna (red), and rrf (ribosomal recycling factor) + trna. How does a site recognise proteins and trna. Proteins have evolved to form proteins that are similar in shape to trna -> cycling/ recyclig factors can bind in that pocket. Release factors have already come in and cleave protein off dna, need to dissemble before .
