CHEM 451 Lecture Notes - Lecture 7: Alpha Helix, Beta Sheet, Stop Codon
Document Summary
Sep/sep = little protein present, less than all other contaminants, dirty, low protein yield. Except from when they get sep/sep they get same amount of protein. With sep/ser = slight band, but sep/sep = clearest band. Mbp proceeds mek, because it always gets made. Lots of product where they don"t read through stop codon. Ser/ser and glu/glu = no premature truncation, just incorporates to folding protein, less dirty. In sep/sep == vast majority in bottom graph is truncation products. B = native sequence in alpha helix and beta sheet where they are trying to substitute. Illustrates how challenging it is to predict structures of protein. Can take same sequence and local environment will influence the type of secondary structures formed. Cyan = designed sequence, based on nmr, forms helix in one part of protein, beta sheet in another part of protein. If something occurs more frequently, higher propensity to be in a specific secondary structure/ region of secondary structure.
