BIOL 2020 Lecture Notes - Lecture 5: Partial Pressure, Structure Formation, Heme
29 Apr 2016
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Document Summary
Amino acids are linked by peptide bonds through condensation reactions (water molecule produced) Polypeptides and peptides can be fragmented by: chemical hydrolysis. Trypsin (basic: arg, lys) -> hydrolyzes c-side peptide bonds. Amino acid sequence determination: n-terminal sequencing. Structural elements held together by hydrogen bonding. -sheets: composed of anti-parallel or parallel strands. Turns ( or hairpin turns) and loops. Can consist of one or more domains: domain: Structurally independent segment of a protein with its own function. Modular proteins: composed of multiple domains in series. Multiple polypeptide chains assemble into a single protein. When folded properly, a protein is said to be in its native conformation. A protein can be denatured through heat, changes to ph or exposure to various chemicals (denaturing agents). The primary structure/ sequence of a protein plays a major role in determining the. Left is functional form of protein; treatment with denaturing chemicals causes denaturation. Now protein is misfolded, non-functional, no activity in terms of hydrolyzing rna.
