CHEM 451 Lecture Notes - Lecture 3: Aminoacyl Trna Synthetase, Aminoacyl-Trna, Stop Codon
Document Summary
-aminoacyl trna synthetases = enzymes that bind the amino acid and trna and they use atp to attach the appropriate amino acid onto the trna. Lots of recognition mechanisms for synthetases (eg. recognise receptor loop stem, in the anticodon loop, variable regions) - use atp to attach a. a to trna. Synthetase is responsible for matching the right a. a and the right trna -> charged trna. If you can train or convince a trna synthetase to attach an unnatural amino acid to a trna, then you have the ability to introduce that unnatural amino acid into your growing protein chain. 2 approaches: 1) site specific 2) global (demonstrated first, came from tirrell, they took the fact that some trna synthetases can"t distinguish from unnatural a. a from natural a. a -> 7&8) 7, 8 = groups could be recognised by synthetase for met. 9, 10 = can be recognised by synthetase for phe.
