CHEM 451 Lecture Notes - Lecture 4: Alanine, Tryptophan, Wild Type
Document Summary
Blue points a = equilibrium, quantify change in folding by tryptophan intensity, measuring wavelength here. Tryptophan changes wavelength/ emission of fluorescent with ____ Bottom of curve at lower wavelength, lower concentration -> folded state inside membrane so less polar membrane. Less polar the environment, shorter wavelength of emission. C 3 state model, b = 2 state model ->the way they fit the model is correct. A is different from b&c as a talks about wavelength, b&c talks about intensity. Can talk about intensity and wavelength and get same results, can see midpoint/ d naturation in both. Significance of alanine in the plot = it"s the native amino acid. Delta delta g is compared to wild type. B-e = different colours represent the different properties of the side chains. Aside from tryptophan, low ph affects aspartic/ glutamic acid. Protonation of aspartic acid will not contribute as much unfavourable energy as you think.
