CHEM 451 Lecture Notes - Lecture 9: Evolutionarily Stable Strategy, Covalent Bond, Equilibrium Constant
Document Summary
Evidence: broadly it"s consistent, but the phi values >1 in nug2/d46a. Phi values > 1 = slowing of unfolding and folding rates, because mutation destabilised transition state more than native state, already distorted native state (?) Mutation that stabilises or destabilises, we talk about changing energies of ts, how you get a. Ts>1 is when you have a mutation that is more destabilising to the ts than it does the native state, this occurs because when the native residue is there it becomes distorted. Greater disruption in the top turn than the bottom bit because the bottom bit is already distorted. Another weird phi value is t11a/wt = -ve values, this means that mutations that are made to nominally destabilise transition state actually stablises transition state instead and destabilises the native state. They have succeeded in their goal generally, phi values are not extrapolated all the way to the axis but they"re generally close to 0 or 1.
