CHEM 451 Lecture Notes - Lecture 8: Protein Folding, Methyl Group, Enthalpy
Document Summary
Rnase t1, people tried to calculate enthalpic and entropic costs of protein folding ot determine stability, this protein is stable but free energy is +9. This means that they have defined free energy state in the opposite direction that we would, folded state expect to be -ve but here they have flipped it around. This is a stable protein but +9 can be confusing. Folded state = -ve, but slide has flipped it around. Flip all the signs, remember g should be -ve to be stabilising. Stabilising contributions should be -ve (but they have it as +ve) Mostly experimental and made a bunch of mutations to find relative contributions of each bit. Number of residues * number of kcal per mole per residue. In the end, thisi s what they came up with -15 kcal/mol determines unstable proteins. We add up small contributions, and the are probably happy that they ended up in similar ballpark relatively close to experimental measurement.
