BME 410 Lecture Notes - Lecture 15: Cell Adhesion, Reactive Oxygen Species, Siloxane
20 Apr 2016
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Addition polymerization: breaking of double bond c=c and attaching to c-c chain, initiation: R-o-o-r breaks to form 2r-0 with free radical. Bond of c-c adds free radical to o free radical: propogation, termination. Two free radicals join together to form final bond. Condensation polymerization: loss of h20 upon addition. Increasing stiffness (kpa) from blood (fluid) to bone. Photoinitiators generate free radicals with uv light: irgacure. 184 and 651 (cid:3) (cid:3) (cid:3) (cid:3) (cid:3) Polyacrylamide gels are not adhesive to cells or proteins. Peg chains must be cross-linked to form hydrogels light, etc) Pdms cross-linking: forms a cross-linked solid elastomer, base, curing (cross-linking agent, hardener=curing agent. Cell adhesion on pdms is low: hydrophobic. Proteins adsorb and denature on hydrophobic surfaces. Fourth state of matter: gas exposed to strong electromagnetic field, gas becomes electrically conductive (ionization) Generate reactive oxygen free radicals and ozone (o3) Pdms oxidized in uv ozone cleaner (cid:3) Both substrates incubated with fibronectin (fn) solution and seeded with cells.
