BME 410 Lecture Notes - Lecture 22: Mutation, Coronary Circulation, Gel
20 Apr 2016
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Result of a blocked left anterior descending coronary artery. Cells are injected: intracoronary, intravenous, intramyocardial, transendocardial. Temperature responsive culture dish: temperature responsive polymer: 37c hydrophobic, <32c hydrophilic, seamless propagation of action potentials. Two cardiac myocyte cell sheets integrate into one tissue (cid:2) (cid:2) (cid:2) Alginate is normally modeled by capacitors but alginate-nanowire has a resistor in its model and close to 0 impedence. Neonatal rat cardiac myocytes, cultures in alginate gels with nanowires formed thicker, more connected tissue compared to those without nanowires. Expression of cardiac markers cx43 (gap junctions) and actinin (sacromeres) were higher in nanowire scaffolds. Cardiac tissue constructs incubated with calcium sensitive dye. Only constructs with nanowires were electrically coupled. Honeycomb scaffolds made from biodegradable polymer (pgs: anisotropic mechanical properties similar to native heart. Decellularized cardiac matrix injected into rat hearts two weeks after myocardial infarction. Two chambered syringe used to deliver gfp esc-derived cardiomyocytes and: glass coverslip poly(n-isopropylacrylamide) polydimethylsiloxane (cid:2) (cid:2) (cid:2) (cid:2) (cid:2)
