BIO 325 Lecture Notes - Reca, Hfr Cell, Plasmid
29 Sep 2013
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Document Summary
Since 1940s, many bacterial genes have been studied in details! Minimal medium - has just the water, a little bit of sugar and mineral salts. Wild-types can grow well on this (prototroph) Auxotroph - lost the ability to synthesize one or more essential compounds, and must be provided with them in the medium if it"s to grow: can"t grow in minimal medium. Additional nutrients must be added in order to grow on the minimal medium. Or you can use supplement minimal medium or complex medium. Selection - growth of the organism under conditions in which only the mutant of interest grows well, whereas the wild type does not. Adaptation hypothesis - proposes that the interaction of bacteriophage and bacterium is essential to the bacterium"s acquisition of immunity to the phage. Spontaneous mutation - occurs in the presence or absence of phage is considered the primary source of genetic variation in bacteria. Mutation usually happens at one loci (doesn"t happen simultaneously)
