Class Notes (807,075)
United States (312,354)
Biology (868)
BIO 325 (43)

BIO 325 September 25th Lecture Notes

5 Pages
Unlock Document

University of Texas at Austin
BIO 325
Sanghamitra Mohanty

9/25 Lecture Wednesday, September 25, 2013 1:58 PM + - + Conjugation between F x F = F F factor is integrated in the bacteria chromosome This type of conjugation can be used for gene mapping with the origin [interrupted mapping --- time map] 2 kinds of F cells:  Hfr  F' o During excision, it has brought tiny amount of bacteria chromosome R plasmid - drug resistance plasmid; can be transferred from one bacteria to another through conjugation Col plasmid - in the E. coli; colicin enzyme RecA and RecBCD proteins Transformation - small pieces of extracellular DNA are taken up by a living bacterial cell and integrated stably into the chromosome  2 events that happen independently o Entry of DNA (may happen)  If it's a plasmid, it doesn't need integration --- will free survive in the cytoplasm since they can auto replicate  But if it's a small strand of DNA and didn't get integrated, then it will be gone o Integration of bacteria DNA into the chromosome  May not happen  Recombinant region contains one host strand (present originally) and one mutant strand  Because these strands are from different sources, this region is referred to heteroduplex  Competent cells - competence is determined by three factors: concentration of the DNA, growth phase of the bacterium, and composition of growth medium o Can be enhanced by several things  Calcium chloride  Electric current  Heat  Increasing concentration of DNA  3 factors that are equal: transport molecules, receptor sites and ATP (active process)   Cotransforming - genes that are close enough to each other to be linked Bacteriophages - infects a host bacterium by injecting their DNA o This DNA serves as a template for the infected bacterium to produce progeny phage particles, which are released when the host cell is lysed (destroyed) o Structure:  The tube/sheath can be compressed  Compression happens through the tube out from its 'bottom'   Lysogeny - the phage DNA integrates into the bacterial chromosome, it's replicated along with the chromosome and is passed to daughter cells o Will do when it senses that the bacteria is surviving in a nitrogen-deficient medium  N is needed to supply nutrients o Prophage - lysogenic bacteria
More Less

Related notes for BIO 325

Log In


Don't have an account?

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.