BIOL 2100 Lecture Notes - Lecture 39: Antisense Rna, Base Pair, Molecular Mass
22 Nov 2016
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Ire control regulated mrna stability (post transcription) and translation. Non-(cid:272)odi(cid:374)g rna"s can work at multiple level, but example focuses on how they influence mrna stability and translation. Experiment with worms, used as model in developmental biology. Trying to inhibit gene expression to find out role of protein. Antisense rna approach, adding this strand to mrna. Mrna is single stranded (coding, sense strand) If want to block translation bring mrna to the cell where antisense strand is, the two strands would pair up a(cid:374)d tra(cid:374)slatio(cid:374) would(cid:374)"t o(cid:272)(cid:272)ur. What results indicated: something was catalytic, small amount of mrna wipes out everything in the cell. Antisense strand expected to have effect, but had no effect. When double strand added, unexpected effect happened. Example of another experiment with similar results. Dou(cid:271)le stra(cid:374)d rna"s i(cid:374)itiate pathwa(cid:455) (cid:271)(cid:455) i(cid:374)tera(cid:272)ti(cid:374)g with protei(cid:374)s. Double strand formed when a strand folds on itself or 2 strands come together.
