BSCI 1511L Lecture Notes - Lecture 3: Powdered Milk, Phenylalanine, Chlorhexidine
5 Apr 2016
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Problem set no. 3- protein quantitation and purification: calculations for concentration of powdered milk solution and the dilutions required to create the 1. 5 mg/ml and 2. 0 mg/ml solutions. As stated in the lab manual, 0. 3 g of powdered milk were administered by the ta. The serological pipette was used to add 10. 0 ml of water, and the mixture was vortexed until completely dissolved. The concentration of the sample was calculated, assuming that the powdered milk took up negligible volume, using the following equations: 0. 3 g powdered milk =104. 35 10. 2. 0 mg /ml diluted 80 10. Volumestock = 15. 333 10-3 ml = 15. 333 ul of stock solution needed. The volume of stock solution needed was determined using the following equation: concentration diluted volumediluted. 1. 5 mg /ml diluted 80 10. 3ml=10. 435 mg / ml stock volume stock . Volumestock = 11. 5 10-3 ml = 11. 5 ul of stock solution needed: absorbance/ concentration data, calculating concentrations of whole and skim milk samples.
