BIOL 1105 Lecture Notes - Lecture 18: Complementary Dna, Recombinant Dna, Selectable Marker
13 Nov 2017
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Contrast the use of selective breeding versus recombinant. Selective breeding uses controlled mating to develop an organism with desired characteristics. Can take many generations to achieve desired results. May result in selection of undesirable/harmful traits along with desirable ones. Typically as a result of the reduction of genetic diversity. Recombinant dna technologies involve the manipulation of dna using enzymatic reactions. Dna sequences from multiple sources can be recombined and inserted into an organism. Can introduce traits that are not native to the organism. Much faster method of trait introduction than selective breeding. Modern corn + toxin = pest resistant (bt) corn. Discuss how endogenous dna replication and repair enzymes. In bacteria, they produce organisms from viruses. Dna ligase is a dna repair enzyme that reforms broken phosphodiester bonds in the backbone of dna. Only enzyme used in cellular dna replication that is required to perform pcr. Explain how dna molecules may be separated by gel.
