BIOL 2604 Lecture Notes - Lecture 5: Hyperthermophile, Absorbance, Methylene Blue
Document Summary
Finish growth dynamics measure growth understand environmental factors: temp, ph, oxygen [5 ways], water. Direct count put it on a slip of known volume with grid, count cells, know total cells/ml. Total cell count: problems, counts both livable and nonviable cells [or starting to die cells] methylene blue can help to differentiate. So no viable numbers at the point at which they are taken: hard to count with motile cells. Sometimes chcl3 added to kill em to make it easy to count. Pipette . 1ml or less on sample, spread evenly with glass spreader incubate and count! Using the factor of dilution in range of 30-300, count colonies and know the amount you started with. [supposed that each colony came from individual cell] *cfu/ml [colony forming units] cuz cells stick together. Pour-plate method sample is pipetted into sterile plate, sterile medium is mixed well.
