BIO 214 Lecture Notes - Lecture 9: Trypticase Soy Agar, Media General, Gelatin

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5 Feb 2017
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Lecture 11
Dr. Fan
January 2nd, 2017
Microbial Growth and Nutrition Continued
ABC Transporters
ATP-Binding Cassette transports
Observed in bacteria, archaea, and eukaryotes
This type of transport is a group effort
ATP converts into ADP and then the nutrients (solute-binding protein)
enter the cell
Types of Microbiological Media
Liquid
Referred to as “broth”
Components (example: nutrients) are dissolved in water and then
sterilized
Semisolid Media
A medium to which has been added a gelling agent
Agar is most commonly used
Gelatin
Chemically Defined Media
Chemically defined media is when the exact chemical composition is
know
Example: when you use minimal media for bacterial genetics
experiments
Minimal media because we know the exact amounts of amino acids to
make growth but we only give the minimum amount needed to obtain
selective growth
Act like you’re putting it on a diet
Complex Media
The exact chemical composition is UNKNOWN
Often contain plant or animal extracts
Example: soybean meal, milk protein, etc.
Include most routine laboratory media
Example: tryptic soy broth
LOTS of nutrients so there is a wide variety of growth
Universal media because there is not selective growth like you have in
minimal media
Some Media Components
Peptones
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