BIO 214 Lecture Notes - Lecture 12: Absorbance, Spectrophotometry, Serial Dilution
Document Summary
The four phases of the growth curve in detail. Cells are adjusting, enlarging, and synthesizing critical proteins and metabolites. They are not doubling at their maximum growth rate during this. Most cases this phase can be very short or even absent. Maximum exponential growth rate of cell division rate of growth is. There is a depletion in nutrients, released waste can inhibit growth. When the number of cells that stop dividing equal the number of cells that continue to divide. The highest growth rate is when the concentration equals 1. 0x10 9. Number of bacteria is at its highest number at this phase. Majority of cells begin to die exponentially due to the lack of nutrients. A chemostat provides a continuous supply of nutrients which will then prevent death phase from ever happening. Based on the cells of microorganisms ability to absorb the light. Measured as optical absorbance in a spectrophotometer. Optical absorbance is directly proportional to the concentration.
