Biology And Biomedical Sciences BIOL 2960 Lecture Notes - Lecture 36: Genome Editing, Alternative Splicing, Chromatin
24 Apr 2017
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Premise: decode the entire set of genetic instructions for an organism = genome. In practical terms = determine the primary base-pair sequence of all the dna in an organism. Then, you will know all the components necessary to support the biological function of a particular organism. Access to all the genes in an organism -- comprehensive database that facilitates numerous medical and basic science applications: Diseases: facilitate genetic marker development for disease prediction and disease gene isolation. Pharmaceutical production: identify genes that encode potential protein products as medicines and/or that are targets of new drugs. Clone by pcr: any sequence immediately accessible by pcr amplification from dna or cdna. Reverse genetic analysis: functional analysis of cell pathways and processes. Microarrays, gene chips, next generation sequencing: many applications including definition of global transcriptional regulatory networks. The human genome project used sanger dna sequencing technology and a slow, expensive strategy called map-based sequencing.
