Biology And Biomedical Sciences BIOL 2960 Lecture Notes - Lecture 37: Reagent, Restriction Enzyme, Homology Directed Repair
14 Aug 2018
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Genomics: determine the dna sequence of all the dna in an organism (genome) then we will know at the components. Dna: purpose: introduce a mutation at specific sequence in the genome, homology directed repair (homologous recombination) An approach for genome sequencing: the determination of the order bases of the genetic information found in an organism. Hierarchical shotgun sequencing: generate a molecular map of large fragment cloned into a bacterial artificial. Chromosome (bac) vectors: these large fragments are further fragmented into smaller pieces and cloned into vectors shotgun cloned , sequence and reassemble these smaller pieces. Cancer cells: 2 heritable properties, they reproduce by escaping normal controls on growth and division, neoplastic new growth, they invade and colonize territories reserved for other cells. When you"re done studying for this unit, you should be able to: decide which of the following genomic techniques would be best suited to answer a given experimental question: microarray, chip-seq, crispr/cas9.
