Biology And Biomedical Sciences BIOL 2960 Lecture Notes - Lecture 9: Cyclic Adenosine Monophosphate, Repressor, Catabolism
26 Mar 2016
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Gene: molecular definition: all information needed to synthesize rna or protein. Coding sequence: open reading frame, orf, if gene encodes protein. Gene: genetic definition: everything required to add back to a mutant to restore function to a mutant (complementation). Basic structure of a typical bacterial protein-coding gene: Open reading frame (orf) = protein coding sequence. Promoter: rna polymerase binds here, determines trx start -10, -35 regions. Prokaryotic genes often organized in operons = multiple orfs present in one transcript (each has own atf, stop) Dna replication, trx and trnsl in one compartment. Rna polymerase binds to dna template: at promoter. One strand is the template: for rna synthesis. For any one gene, only one strand is the template. Rna is synthesized from rntps, 5" to 3" direction. Form base pairs, complementary to the bases on dna template. 5" end of transcript is displaced from template as polymerase moves. Alpha: enzyme assembly, promote interaction with regulatory proteins.
