Biology And Biomedical Sciences BIOL 2960 Lecture Notes - Lecture 12: Transesterification, Exon, Cyclic Adenosine Monophosphate
18 Apr 2016
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Both steps in splicing are classified as transesterification reactions, where a phosphodiester bond is broken and remade. Both steps require enzyme activity of snrnps, u1, u2, u4, u5, and u6. An example of cell-type specific alternative rna processing -- two different proteins from a single primary transcript. High levels of cgrp may cause migraines, binds to gpcr to elevate camp in target cells. Calcitonin decreases blood calcium, binds to a different gpcr. Boxes: sequences which are defined as exons by the splicing machinery and are included in a mrna. Lines: sequences seen as introns and spliced out, not seen in the mrna. Exons are defined by protein complex (u2af) and u2snrnp, and u1snrnp respectively, supplemented by exon splicing enhancer elements. Exons are defined by interactions between proteins bound to 3" and 5" splice sites and exon- bound proteins, the 5" cap, and the polya signal. 1st 5" exon: starts at transcription start site (5" cap)
