L41 BIOL 2970 Lecture Notes - Lecture 6: Interphase, Trypsin, Wild Type
2 Nov 2016
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If genes are very far from the centromere, (cid:1151) of them will have 1st division segregation and (cid:1152) of them will have 2nd division segregation. 3000 * 10^6 bp in genome, so 3 million bp differ between 2 haploid genomes. Polymorphisms: at least 2 common forms in population. Two frequent types of polymorphisms in sequence: single nucleotide polymorphism (snps, insertions or deletions of a small # nucleotides (indels) Founder effect: alteration in frequency of an allele due to founding a population by a small number of individuals. Mice expressing 100 amino acid n-terminal fragment of hd protein with 150 cag. 9 other genes in which expansion of cag repeats causes neural degeneration. Hd gene encodes a protein of 3144 amino acids. Hairpin (caused by duplication) is resistant to cleavage by fen. Each spat has a 25 base long oligonucleotide. For 1 individual: dna is labelled with red fluorescent dye.
