L41 BIOL 4810 Lecture Notes - Lecture 27: Teichoic Acid, Ribitol, Lipopolysaccharide
12 May 2020
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Document Summary
Bacterial lipopolysaccharide: o-specific chain located in outer surface of the bacteria, lipid a anchors the molecule to the membrane, found in the outer membrane of gram-negative bacteria, different sugar structure in o-specific chain confers immunoreactivity. N-linked glycosylation: typically a nag is -linked to the amide nitrogen of an asparagine, found in: asn-x-ser or asn-x-thr (x is any aa but pro, and rarely asp, glu, leu, or. Trp: typically have a branched (mannose)3(nag)2 core, these residues can be linked to other sugar residues, making for highly diverse possibilities, core pentasaccharide: Diversity in n-linked glycosylation: complex mixtures are formed, the pentasaccharide core is kept constant and made from trimming a precursor. Bacteria initiate infections by attaching to host cells via adhesins. Glycobiology: experimental tools are extremely limited, metabolically label glycans with synthetic azidosugars and image, glycoproteomics, glycosylation patterns are highly correlated with malignancy, metabolically label classes of glycoproteins with azidosugars and then identify them with mass spectrometry.
