L07 Chem 151 Lecture 16: Spring Experiment 7 Lecture
29 Mar 2017
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Chromatography method of separation based on phase distribution. Stationary phase solid particles/liquid coating on solid particles. Mobile phase liquid or gas carrying the sample mixture. Carrier (eluent) liquid or gas carrying the sample mixture. Elution mobile phase moving over the stationary phase. Thin layer (stationary phase is spread very thin) Column chromatography stationary phase packed into a column containing ion exchange resin. Never let liquid level drop below the top of the resin. Greater affinity for stationary phase results in slower travel through column. As column drains, compounds will separate based on affinity. Dowex-50 polymer beads attached to sulfonate groups (weakly bonded to protons) When metal cation passed through column, h+ displaced. Greater + charge, greater affinity of cation for resin i. e. m3+ > m2+ > m+ > h+ in terms of affinity. Separated ions can be collected because eventually all cations will elute.
