AAH 3002 Lecture 1: Imiphumela yokufunda
Document Summary
Ekupheleni kwale vidiyo, abafundi kufanele bakwazi: ukuqonda futhi ukwazi ukuchaza izimiso ze-fluorescence, chaza umehluko phakathi kwemicroscope evamile ne-fluorescence microscopy qonda ukusebenza kwangaphakathi kwesibonakhulu se-fluorescence. Ukukhipha enye yezinsalela ezishajelwe kahle (lys -->thr kulesi sibonelo) kulesi siginali yokungenisa i-nuclear (ebuye ibizwe ngokuthi isignali yendawo ye-nuclear noma i-nls) ngokuguqulwa kwanele ukuvimbela ukwenziwa kwasendaweni kwe-nuclear ngisho noma amanye amaprotheni ahlale engashintshiwe. I-fluorescence microscopy: i-fluorescence impahla yokukhanya okumunca amaza amaza amafushane kanye nokukhanya okukhiphayo kwamaza amade amade, ukukhanya kumuncwa i-molecule ye-fluorescent eyaziwa ngokuthi i-fluorophore, i-fluorophore iyikhemikhali ye-fluorescent engaphinda ikhiphe ukukhanya lapho kubangwa ukukhanya. Amandla amuncwa i-athomu eba nesasasa: i-electron igxumela ezingeni eliphezulu lamandla, i-electron iwela emuva esimweni esiphansi, ikhipha i-photon (noma iphakethe lokukhanya) - i-athomu i-fluorescing. I-microscopy ye-fluorescence: iyithuluzi lokuhlaziya elinamandla elihlanganisa izici zokukhulisa i-microscopy ekhanyayo ne ukubonakala kwe-fluorescence, kufezwa kusetshenziswa isibonakhulu sokukhanya esiyisisekelo ngokuhlanganisa ukusetshenziswa komthombo wokukhanya onamandla, izihlungi ezikhethekile, kanye nezindlela zokulebula ngokukhanyayo isampula.
