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Please show how the following would be prepared?
Pipette 1 µg of total RNA into a 1.7 ml RNAse-free microfuge tube. Add the appropriate amount of loading buffer plus SYBR Green; the stock solution is 6X (final concentration should be 1X). If needed, adjust volume with DEPC-treated water so that the final volume is 10 µl
1) 4L1X Transfer Buffer: 25mM Tris, 192mM Glycine, 20%(v/v) Methanol, 0.1%(w/v) SDS
Calculate the volume amount of 10X Transfer stock solution to dilute to 1X concentration in 1L. Calculate the volume amount of methanol to make a final concentration of 20% (v/v) and the volume amount of 10% (v/v) SDS to make a final concentration of 0.1% (v/v)
What volume must I add to 3 mL of 300 micrograms/mL stock if I wish to obtain a final concentration of 20 micrograms/mL? What is the calculated dilution factor?
