BTNY 11000 Lecture Notes - Lecture 8: Agarose Gel Electrophoresis, Electrophoresis, Plasmid

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Lab exercise 7 mitosis & molecular biology. Review textbook chapter 16: gel electrophoresis of plasmid dna. Genetic engineering introduces dna changes in order to produce a desirable or beneficial characteristic in the resulting organism. On a changing planet and with humankind"s increasing need to explore beyond earth, agriculture needs to be able to meet challenges including an ever-changing climate, disease threats to monocultures and adaptability to non-earth conditions. One technique of molecular biology allows researchers to use small, circular pieces of bacterial dna (called plasmids) in order to manipulate and introduce new dna into plant cells. Dna segments added, put back together and inserted into plant cells to introduce beneficial traits. The circular plasmid dna is cut open by a restriction enzyme (fig. 16-10) at a very specific sequence of dna. New, introduced dna is made to match that specific sequence so the new dna fits into place within the plasmid.

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