BIO SCI 97 Study Guide - Cas9, Endonuclease, Bacteriophage
25 Dec 2022
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Document Summary
A method that bacterias use to protect themselves from phage infection >>> transduction. 1] tracrrna + crrna >>> become guide rna. 2] double strand breaks are caused when sequences are cut by cas9. 3] dna can be fixed through homologous recombination. Introduction of a new dna sequence from an inserted donor dna. 4] mutations can occur at the double strand break. Non-homologous ends join >>> excess or loss of dna. Tandem repeat (vntr) using pcr (vntr) using pcr. Variable number tandem repeat (vntr): sequences of 10+ base pairs each that are variable. Restriction fragment length polymorphism (rflp): a fragment of. Dna generated by treatment with a restriction endonuclease. Restriction endonucleases: enzymes that recognize and cleave specific sequences of dna >>> restriction sites. Single-nucleotide polymorphism (snp): a single base pair difference in a specific genome location detected by comparing individual dna sequences. Can easily be detected if it creates or destroys a restriction site.
