BIOTECHNOLOGY Study Guide - Final Guide: Alkaline Phosphatase, Aspergillus Nuclease S1, Ribonuclease H

In prokaryotes, DNA is protected from restriction enzymes by the action of

3. Listed below are several restriction enzymes along with specific nucleotide sequence recognized by the restriction enzyme:

Restriction enzyme Recognition sequence

AluI 5’-AGCT-3’

HaeIII 5’-GGCC-3’

HindIII 5’-AAGCTT-3’

BamHI 5’-GGATCC-3’

NotI 5’-GCGGCCGC-3’

Let’s assume that you would like to digest chromosome 1 from the fruit fly Drosophila with these restriction enzymes.

For each of the above enzymes, if we assume that the Drosophila genome is composed of 30% A, 30% T, 20% G, and 20% C, what is the average distance between each of these recognition sequences?

1. What are restriction enzymes and what are they used for inmolecular biology?