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BIOC 2580 Lecture Notes - Lecture 2: Size-Exclusion Chromatography, Ovalbumin, Elution

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juliastreet1212
19 Apr 2023
School
University of Guelph
Department
Biochemistry
Course
BIOC 2580
Professor

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Document Summary

Proteins write-up: plot a chromatogram with absorbance readings on the y-axis and elution volume on the x-axis, giving proper figure title and label the axis. Indicate which fraction(s) have the highest concentration of. Nadh and which have the highest concentration of ovalbumin. Ovalbumin and what is the elution volume for nadh. (3 marks) Figure 1: absorbance measurement for ovalbumin at 585nm and for nadh at 340nm for 15 fractions collected from the gel filtration column. A 200 l mixture of ovalbumin and nadh were added to the column using a micropipette and eluted with 50mm of phosphate buffer at a ph of 7. Overall, 15 fractions were collected with 15 drops per fraction, each fraction being equivalent to 1ml. An aliquant of each fraction was collected to measure at 595nm and 340nm after adding the bradford dye reagent. The highest concentration of ovalbumin was observed at fraction 4 and the highest concentration of nadh was observed at fraction 9.

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