the question is
You are provided with a 1 cm3ofbiological sample from which 0.1 mm thin sections are cut.Asearch for virus cells at a magnification of 100,000 hasbeenproposed. The fluorescent screen on which the image is tobeprojected has a diameter of 15 cm.(
a) How many fields needtobe searched to cover an area of 0.1 mm2?
(b) Whatpercentage of the original sample does the searchedvolumerepresent?
(c) Commenton the significance of the calculated values.
3. The attempt at a solution
I dont know of any formulas for this question.
The magnification might be a key here.
Can I find the resolution of the TEM using this?
or maybe the max distance squared I get at 100000 mags (onefield)and then use that to calculate the total number of fields tocover0.1 mm2 ?
In the second part I can do the same (find the area at 100000mags)and get the percentage.
thanks.
the question is
You are provided with a 1 cm3ofbiological sample from which 0.1 mm thin sections are cut.Asearch for virus cells at a magnification of 100,000 hasbeenproposed. The fluorescent screen on which the image is tobeprojected has a diameter of 15 cm.(
a) How many fields needtobe searched to cover an area of 0.1 mm2?
(b) Whatpercentage of the original sample does the searchedvolumerepresent?
(c) Commenton the significance of the calculated values.
3. The attempt at a solution
I dont know of any formulas for this question.
The magnification might be a key here.
Can I find the resolution of the TEM using this?
or maybe the max distance squared I get at 100000 mags (onefield)and then use that to calculate the total number of fields tocover0.1 mm2 ?
In the second part I can do the same (find the area at 100000mags)and get the percentage.
thanks.
