MCB 2050 Study Guide - Northern Blot, Frameshift Mutation, Southern Blot

• School: University of Guelph
• Department: Molecular and Cellular Biology
• Course: MCB 2050
• Professor: Jim Kirkland

Document Summary

20. 1 operons are common in bacteria but not in eukaryotes. Ans: in multicellular eukaryotes, the environment of an individual cell is relatively stable. There is no need to respond quickly to changes in the external environment. In addition, the development of a multicellular organism involves complex regulatory hierarchies composed of hundreds of different genes. The expression of these genes is regulated spatially and temporally, often through intricate intercellular signaling processes. In bacteria, translation of an mrna begins before the synthesis of that mrna is. Ans: coupling of transcription and translation is not possible in eukaryotes because these two processes take place in different cellular compartments transcription in the nucleus and translation in the cytoplasm. 20. 3 muscular dystrophy in humans is caused by mutations in an x-linked gene that encodes a protein called dystrophin. Another technique would be to hybridize dystrophin rna in situ that is, in the cells themselves with a probe from the gene.