BIEB 166 Lecture Notes - Lecture 7: Fyn, Knockout Mouse, Transgene
8 Nov 2013
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Block ltp with drugs (inhibitors) rats have decreased ability to learn spatially. Too general to block their learning ability alpha camkii mutants. Silva, a. j. et al. (tonegawa lab at mit) science 257: 206 (1992) Strengthening of connections (synapses) between nerve cells involved in memory. Hippocampus contains a kinase (alpha camkii) that controls spatial learning of the mice. Water-type mice take less time to reach the platform. Mutant mice largely normal but inferior at the hidden platform test b. Spatial learning in fyn tyrosine kinase knockout mice. Mayford, m. et al. (kandel lab at columbia) science 274: 1678 (1996) a. b. c. Forebrain specific promotor combined with tetracycline transactivator system (feeding tetracycline temporarily suppresses camkii) Provided both regional and temporal control of transgenic expression. Could use this promotor to switch alpha camkii transgene on and off in adult animal by feeding it tetracycline. A change in behavior as a result of experience.
