BIO206H5 Lecture Notes - Lecture 9: Caat Box, Tata Box, Eukaryotic Transcription

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1 Dec 2013
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Larger genome more genes to recognize and transcribe. Dna organization: tightly condensed chromatin with many proteins. Termination site trimming site where rna molecule gets cut down. Coding region (exons and introns) exons contain information that will be translated. Introns are intervening process that will be removed. Initiation binding of rna polymerase to a promoter sequence. Elongation sequential addition of ribonucleotides as prescribed by the dna template strand. Termination dissociation of rna polymerase (remove from dna) release of primary transcript from template. Initiation of eukaryotic gene transcription is a complex process. 3 rna polymerase: rna polymerase i, rna polymerase ii, rna polymerase iii. Rna polymerase i: transcribe larger rrnas (28s, 18s, 5. 8s) Rna polymerase ii: mrnas, most small nuclear rnas (snrnas and snornas), most micrornas, and telomerase rna. Rna polymerase iii: small rnas, including trnas, 5s rrna, and u6 snrna. Rna polymerase iv, v (plants only): sirnas (silencing genes)

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