BIO-0013 Lecture : Bio 13 Lecture 30 Notes.docx
Document Summary
How does e. coli choose glucose over lactose when both are available: presence of glucose suppresses the production of the enzyme that breaks down lactose, so there is no way that lactose can be used, must use glucose camp is synthesized when glucose levels are low, atp is converted to cyclic amp and two phosphates via adenylyl cyclase, glucose inhibits the activity of adenylyl cyclase wont be able to make camp, low a. c. and low camp so low transcription of the lac operon, when you have low glucose, high a. c. and high camp so high transcription of lac operon, cap = catabolite activator protein site (on dna, regulatory sequence, promoter for lac operon does not have a big consensus sequence so it doesn"t have lots of room for binding and sigma factors not a great promoter, gets help from cap binding site, when you have lots of camp (low glucose) it binds to cap.