BIOL 2324 Lecture Notes - Dithiothreitol, Hindiii, Sodium Chloride

• School: Northeastern University
• Department: Biology
• Course: BIOL 2324
• Professor: Thomas Manning

Document Summary

Plasmid dna is cleaved fragments of dna are then mixed back circular dna that can be used as a vector by together or mixed with other dna fragments with inserting specific dna sequences and transferring complementary base pairs, they can be recombined the dna to a host cell. 1, 2, 5, 6 this is the basis for a great deal of biochemical research. 1mm dithiothreitol, ph7. 5: distilled water, hindiii restriction and then gel electrophoresis was carried out on a enzyme, pvuii restriction enzyme, bgli restriction enzyme. From standard curve; y= 0. 2538x+4. 9294 x= 5. 6 x= 5. 8 x= 5. 9 x= 6. 2 y= 0. 2538(5. 6) + 4. 9294 y=3. 5081. Discussion x= 6. 8 x= 7. 0 x= 7. 4 x= 7. 5 x= 8. 0 x= 9. 4 x= 9. 5 x= 9. 8 x=12 y= 0. 2538(6. 2) + 4. 9294 y=3. 3558. Hind iii and bgl i (cid:224) (cid:224) (cid:224) (cid:224) (cid:224) (cid:224) (cid:224) (cid:224) (cid:224) (cid:224) (cid:224) (cid:224) (cid:224) (cid:224) (cid:224) (cid:224) (cid:224) (cid:224) (cid:224) (cid:224)