BIOL 3096 Chapter Notes -Copi, Lead Compound, Copii
3 Mar 2014
School
Department
Course
Professor
Document Summary
Following the radio-labeled glucose (pulse) is a whopping dose of non-radio-labeled glucose (chase). This allows the scientist to see the pulse clearly: the animal"s body itself acts as a natural chase. but the added non-radio-labeled glucose dilutes the pulse even more. Introduce a gene for gfp, use microscopy to visualize movement of label: to reduce amount of fluorescent protein, use a temperature-sensitive mutant, golgi apparatus is quickly labeled and fluoresces intensely. Frap- fluorescence recovery after photobleaching: a variation of the previous technique, an area is photobleached and then the time interval over which fluorescence returns to that area is monitored. In the example, the golgi apparatus is photobleached and allows one to clearly see the fluroescent proteins that are traveling to the golgi apparatus, restoring its fluorescence. 10/21/13: these techniques allowed for the development of an image of the pathway of vesicles from the.
